Development and validation of HPLC method for estimation of propranolol HCl in human plasma

This study presents a rapid, sensitive and accurate high performance liquid chromatographic (HPLC) method for determination of propranolol HCl in human plasma. Human plasma samples were subjected to protein precipitation by methanol and protein free plasma samples were directly injected into HPLC C18 column. Mobile phase consisting of a mixture of acetonitrile: pH 4.5 phosphate buffer (35:65) was delivered at a flow rate of 1 ml/min. A good separation of propranolol HCl and diltiazem HCl as internal standard was achieved with retention times of 6.6 min and 9.9 min respectively. Linearity was obtained for propranolol (conc. range 20-280 ng/ml) and followed regression equation, Y= 0.0162 X – 0.0515. Peak areas were reproducible as indicated by low coefficient of variation (< 2.13%).